100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50ml of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant.
Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature.
Primary antibody was diluted in blocking buffer to the appropriate working dilution.
Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with antibody.
After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies.
After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：150ul
Host：guinea pig
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Specificity：Recognizes rat ASIC3.
Isotype：IgG
Calc Applications Abbrev：IC IHC
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide (C-TASLDPDDFDPEPSDPLGSP) residues 285-304 from the extracellular domain of rat ASIC3.
