84-1050-18　Rhodopsin, Red Green (OPSD, Opsin 2, Opsin 2 Rod Pigment, Opsin-2, OPN2, Retinitis Pigmentosa 4, Retinitis Pigmentosa 4 Autosomal Dominant, RP4) 100ug　R1998-18

特徴

• The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms.
• Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum.
• All three opsins are transmembrane proteins with seven membrane-spanning regions.
• Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation.
• Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992).
• The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin.
• Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: 1:200 on formalin-fixed, paraffin-embedded mouse retina tissue.
• Staining with citrate buffer, pH 6.0 is required
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.