S-Adenosyl-I-methionine-dependent protein arginine N-methyltransferases (PRMTs) methylate arginine residues within a variety of proteins, particularly histones.
At least four distinct mammalian family members have now been described, including PRMT1, PRMT3, CARM1/PRMT4 and JBP1/PRMT5
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100-1:500.
Reacts with a band of ~57kD on whole HL60 cell lysate.
Additional bands may be present depending on sample used
ELISA (direct): 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:400ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified immunoglobulin
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:Recognizes human PRMT3 (Protein Arginine N-Methytransferase 3).
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide from N-terminus of human PRMT3.