The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite outgrowth.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes human Ser144 phosphorylated PAK1 and Ser141 phosphorylated PAK2. This antibody also recognizes Ser139 phosphorylated PAK3 but does not cross-react with phosphorylated PAK4-6. Species Crossreactivity: guinea pig. Species sequence homology: rat and mouse -100%.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Gp Hu Mo Rt
Immunogen：Synthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1.
