N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors.
NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning.
NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B).
The NR2 subunit acts as the agonist binding site for glutamate.
This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes rat NMDAR2B phospho Tyr1472. The antibody recognizes a protein of ~180kD in Western blot. The labeling of the NMDAR2B protein in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. Two additional lower molecular weight bands are present in Western blots of rat brain.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to amino acids surrounding the phospho Tyr1472 of rat NMDAR2B.
