Gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the brain, mediates neuronal inhibition by binding to GABA receptors.
The type A GABA receptors are pentameric chloride channels assembled from among many genetic variants of GABA(A) subunits.
This gene encodes the gamma 2 subunit of GABA(A) receptor.
Mutations in this gene have been associated with epilepsy and febrile seizures.
Alternative splicing of this gene results in transcript variants encoding different isoforms.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunopreciptation: will immunoprecipitate ~50% of all GABAA receptors in SDS extracts of rat forebrains (150ul) using 15ug of antibody
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes rat GABAA receptor gamma2 subunit. Reacts with the ~44-47kD gamma2 subunit of the GABAA receptor in rat brain membrane fractions.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Rt
Immunogen：MBP fusion protein corresponding to the cytosolic loop of rat GABAA receptor gamma2 subunit.
