The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system.
This gene encodes GABA A receptor, beta 2 subunit.
It is mapped to chromosome 5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2 subunits of the GABA A receptor.
Alternative splicing of this gene generates 2 transcript variants, differing by a 114 bp insertion.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunopreciptation: G1015-18L will immunoprecipitate ~70% of all GABAA receptors in SDS extracts of rat forebrains (150ul) using 15ug of antibody
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes rat GABAA receptor beta2 subunit., specific for the ~50-56kD in rat brain membrane fractions. Species Crossreactivity: bovine, human and mouse
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：MBP fusion protein from the cytosolic loop of rat GABAA receptor beta2 subunit.
