84-1050-01　Actin, gamma 100ul　A0760-45

特徴

• Actin is a 43kD highly conserved structural protein.
• There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group.
• The majority of the isotype heterogeneity is located in the amino-terminal 30aa.
• The different actin isotypes have been shown to behave very differently in vitro and in vivo.
• Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins.
• There are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000.
• Non-specific bands can occur when A0760-45 is overloaded.
• Blocking with purified milk proteins preferred over commerical concentrated milk solutions.
• Detects a 42-43kD bandImmunocytochemistry: 1:500-1:2000.
• Cultured cells fixed with PFA, then permeabilized with methanol
• Immunohistochemistry: 1:500-1:2000.
• Tissues frozen and then fixed with PFA followed by Triton permeabilisation.
• 10% buffered formalin fixed tissues have also been usedOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.