84-1049-96　Prothrombin 1mg　P9115-09A

特徴

• Prothrombin (factor II, F.II) is a vitamin K-dependent glycoprotein produced in the liver.
• The concentration of prothrombin in plasma is ~100ug/ml (~1.4uM).
• Prothrombin is a single chain molecule with a molecular weight of 72kD.
• Prothrombin consists of a catalytic domain followed by two kringle structures and an N-terminal domain containing 10 g-carboxy-glutamic acid (gla) residues.
• These gla residues allow prothrombin to bind to membranes that contain acidic phospholipids in a calcium dependent manner.
• The binding to membranes is required for effective presentation of prothrombin as a substrate for activation by the prothrombinase complex, which consists of activated factor X, activated cofactor V and calcium on phospholipid membrane.
• Activation by prothrombinase occurs by sequential cleavage after residue Arg320 then after Arg271 to produce the active protease a-thrombin (37kD) and the byproduct prothrombin fragment 1.2 (35kD).
• The product thrombin further cleaves prothrombin fragment 1.2 after residue Arg155 into individual prothrombin fragments 1 and 2.
• The activity of a-thrombin in plasma is inhibited primarily by antithrombin and the rate of inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin.
• Other physiological inhibitors of thrombin in the absence of heparin include a2macroglobulin and a1antitrypsin (1-3).
• Factor II also plays a role in maintaining vascular integrity during development and postnatal life.
• Mutations in Factor II leads to various forms of thrombosis and dysprothrombinemia.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.