In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter.
They consist of two major components in equal amounts, DNA and the basic proteins called histones.
Histones are believed to be regularly arranged in the deep grove of the DNA helix.
The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible.
This allows for the supercoiling of the chromatin fibers.
Histone 2 is rich in lysine.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：sheep
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity：Recognizes bovine Histone 2B. Species crossreactivity: All other DNA containing species.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo
Immunogen：Intact calf Histone 2B complexed to rRNA.
