Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilutions:ELISA: ~0.1ug/well of rhIL-8 can be detected using this polyclonal antibody at a concentration of 2.6ng/ml Western Blot: concentration of 260ng/ml will allow visualization of approximately 5ug/lane of rhIL-8 Neutralization: Total chicken IgY fraction at the concentration of 50ug/ml showed 60% inhibition of 10ng/ml IL-8 induced IL8RB/293 cell migrationOptimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1mg
Host:chicken
Source Antibody:human
Grade:Purified
Purity:Purified Serum
Form:Supplied as a liquid in PBS, pH7.3. No preservative added.
Specificity:Recognizes human Interleukin-8 (IL-8) Exhibits no detectable crossreactivity with human serum albumin, bovine serum albumin and other cytokines tested such as IL-1B, Il-16, TNF-a, GM-CSF, MCSF, bFGF and MIP-1a.
Isotype:IgY
Calc Applications Abbrev:E N WB
Calc Crossreactivity:Hu
Immunogen:Highly purified recombinant human Interleukin-8 (IL-8)