WNK3 (partial 1450-aa, chromosome Xp11.21-23) is a serine-threonine kinase with a catalytic domain which is ~93% homologous to that of hWNK1.
The conserved catalytic lysine in subdomain II is replaced by a cysteine but another lysine residue in the glycine ribbon of the subdomain I confers the WNK3 kinase activity.
Eleven out of 33 residues in the subdomains I and II of WNK3 are conserved in the 18 WNK-related kinases.
The WNK3 gene is predominantly expressed in brain.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Peptide sequences from other species are not yet available. No significant sequence homology is seen with other WNKs or proteins. Antibody reactivity in various species is not established. The control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 16-aa peptide sequence, mapping near the C-terminus of human WNK-3 (1) synthesized, coupled to KLH
