84-1049-79　WNK1 (Kinase deficient protein, Protein kinase lysine deficient 1, Protein kinase with no lysine 1, Serine/threonine protein kinase WNK1) 100ul　W2996-02

特徴

• Protein kinases represent a superfamily of over 400 members that share a catalytic domain of 250-300 amino acids.
• A conserved lysine, in subdomain II of the catalytic core of all 390 protein kinases, anchors a and b phosphoryl groups of ATP.
• The catalytic domain of WNK (With No K=Lysine) kinases contains all invariant residues, except for this lysine residue.
• There are 4 (identified) WNK genes (WNK1, WNK2, WNK3 and WNK4) in human (h) and rat (r).
• The hWNK kinases exhibit ~30% seq identity and 50% seq homology to human Mitogen Activated Protein kinases (MAPK) STK2, PAK2, MEKK3, PAK3 and Raf-1.
• The mutation in genes expressing WNK1 (chromosome 12p13.3) and WNK4 (chromosome 17q) causes Pseudohypoaldosteronism type II (PHAII), an autosomal disorder featuring hypertension, hyperkalemia (high serum K+ level), normal/elevated aldosterone level, increased renal salt absorption, and impaired K+ and H+ excretion.
• WNK1 (251kD; 2382aa, chromosome 12p13.3) is a serine-threonine kinase in which the conserved catalytic lysine in subdomain II is replaced by a cystine but another lysine residue in subdomain I confers the kinase activity.
• The hWNK1 is ~86% identical to rat WNK1(230kD, 2126aa).
• The two transcripts of hWNK1 (8.5 and 10 kb) enzyme are expressed in most tissues but predominantly in kidney, heart and muscle and weakly in brain.
• In cells of these tissues, WNK1 is present throughout the cytoplasm.
• WNK1 localizes to the distal convoluted tubule (DCT), medullary and cortical collecting ducts (MCD and CCD), adjacent segments of the distal nephron that play a key role in salt, water, K+ and pH homeostasis.
• In the CD, Na+ is reabsorbed by the epithelial sodium channel (ENaC) providing the electrical diving force for K+ and H+ secretion.
• Considering the PHAII-features, WNK1 possibly, increases the transcellular or paracellular Cl- conductance in CD, thereby increasing salt reabsorption and intravascular volume, while concomitantly dissipating the electrical gradient and diminishing K+ and H+ secretion.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL).
• WNK1 is ~230-kDELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
• Optimal dilutions to be determined by the researcher.
• Control Peptide: W2996
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.