84-1049-78　WNK1 (Kinase deficient protein, Protein kinase lysine deficient 1, Protein kinase with no lysine 1, Serine/threonine protein kinase WNK1) 100ug　W2996-01

特徴

• Protein kinases represent a superfamily of over 400 members that share a catalytic domain of 250-300 amino acids.
• A conserved lysine, in subdomain II of the catalytic core of all 390 protein kinases, anchors a and b phosphoryl groups of ATP.
• The catalytic domain of WNK (With No K=Lysine) kinases contains all invariant residues, except for this lysine residue.
• There are 4 (identified) WNK genes (WNK1, WNK2, WNK3 and WNK4) in human (h) and rat (r).
• The hWNK kinases exhibit ~30% seq identity and 50% seq homology to human Mitogen Activated Protein kinases (MAPK) STK2, PAK2, MEKK3, PAK3 and Raf-1.
• WNK4 (human 1243-aa, 17q21) is a Ser-Thr kinase in which the conserved catalytic lysine is replaced by a cysteine but another lysine residue in subdomain I confers the kinase activity.
• WNK4 is 76% identical to WNK1 in kinase domain.
• WNK4 is expressed predominantly in kidney, colon and skin.
• WNK4 localize to the distal convoluted tubule (DCT) and cortical collecting duct (CCD), that play a key role in salt, water, K+ and pH homeostasis.
• WNK4 is present exclusively in the intracellular tight junctions in DCT and in both cytoplasm and tight junctions in CCD.
• In DCT, salt reabsorption is mediated by the electroneutral Na-Cl cotransporter.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-1:100,000.
• Control peptide can be used to coat ELISA plates at 50-100ng control peptide/wellWestern Blot: 1:1000-5000 (Chemiluminescence)Optimal dilutions to be determined by the researcher.
• Control Peptide:W2996: WNK1, Human, Control Peptide (With-No-Lysine Kinase 1)
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.