Recently, cDNA cloning by functional expression has identified two homologous but distinct VMAT genes from rat, bovine and human adrenal glands.
VMAT1 (previously termed CGAT for chromaffin granule amine transporter) is primarily expressed in adrenal glands, and it displays low sensitivity to inhibition by TBZ.
It has about 3-fold lower affinity for most monoamines and about 100-fold less for histamine than the VMAT2.
VMAT1 also has a lower turnover number than VMAT2.
VMAT2, previously termed SVAT (synaptic vesicle amine transporter), is primarily found in monoaminergic cell bodies of the central nervous system and also in stomach but not in adrenals.
Tetrabenazine and psychostimulants such as methamphetamine inhibit VMAT2 much more potently than VMAT1.
Thus the VMATs show considerable differences in physiological and pharmacological properties.
VMATs are predicted to contain 12 membrane spanning domains with a large hydrophilic loop and N-glycosylation sites between domain I and II.
Both N- and C-terminus are predicted to be cytoplasmic.
Rat VMAT1 is a 521aa transmembrane protein with 12 transmembrane domain (1).
Rat VMAT2 is 515aa.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100,000.
Using 50-100ng control peptide/well.
Western Blot: 1-10ug/ml using ECL
Optimal dilutions to be determined by the researcher.
Control Peptide:V2121-88G: VMAT2, Control Peptide, Rat
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes rat VMAT2. Does not recognize VMAT1 in transfected cells. Species Sequence Homology: human, 92% (12/13aa)
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 13aa near the C-terminal, cytoplasmic domain of rat VMAT2 (KLH).
