Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
Detects a major band at ~55 kD in postnuclear supernatants of CHO transfected with VMAT1 and not in wild type cells (2).
Some additional bands, both higher and lower MW, were also detected and remain unaffected by the inclusion of protease inhibitors
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human Vesicular Monoamine Transporter 1 (VMAT1). Species Sequence Homology: rat: 63%. No significant sequence homology with VMAT2 or other transporters.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 20aa peptide sequence near the C-terminal, cytoplasmic region of human VMAT1.
