Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
A band of 95-110kD has been detected in brush border membrane (2)
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Affinity purified over the control-peptide Sepharose
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes rat VMAT1. Does not recognize VMAT2 in transfected cells (1, 2). The C-terminal rat VMAT1 sequence does not show any appreciable homology with hVMAT1 (4). Antibodies to rVMAT1 are not expected to crossreact with hVMAT1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A 12 AA peptide sequence near the C-terminus of rat VMAT1 (1). This peptide is predicted to be cytoplasmic. The peptide was synthesized with amino terminal Cysteine, coupled to KLH.
