84-1049-71　［取扱停止］VGAT (solute carrier family 32 (GABA vesicular transporter) member 1, GABA and glycine transporter, SLC32A 1, Solute carrier family 32 member 1, VGAT, VIAAT, Vesicular GABA Amino Acid Transporter, Vesicular GABA transporter, Vesicular inhibitory amino acid transporter, bA122O1.1, hVIAAT, solute carrier family 32 (GABA vesicular transporter) member 1) 100ul　V2121-87X

特徴

• Classical neurotransmitters are synthesized in the cytoplasm, and so must be transported into synaptic vesicles for regulated exocytosis of vesicles filled with neurotransmitter. Although the vesicular transporters for monoamines and acetylcholine have been identified, the proteins responsible for packaging the primary inhibitory and excitatory transmitters, gamma aminobutyric acid (GABA) and glutamate have only recently been identified. Studies in the nematode Caenorhabditis elegans have implicated the gene unc-47 in the release of GABA. Recently, rat homolog of unc-47 have been cloned and characterized. Rat VGAT, a 525 aa membrane protein with ten transmembrane domains, is expressed by central GABA neurons. Both N and C-termini are predicted to be cytoplasmic. Rat VGAT and unc-47 share ~38% sequence identity. Rat VGAT is primarily expressed in the brain
• Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.