Acetylcholine transferase synthesizes the neurotransmitter acetylcholine (ACh) from choline and acetyl CoA.
ACh is packaged and stored into synaptic vesicles until its release.
ACh is hydrolyzed by acetylcholine esterase (AChE) to yield choline and acetate.
Choline is taken up by the high affinity VAChT/VAT located at the membrane and recycled for synthesis of ACh
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:V2121-86C: Vesicular Acetylcholine Transporter, Rat, Control Peptide (VAChT)
Recommended Secondary Antibodies:I1906: IgY Pab Rb x ChI1906-01: IgY, Fc (HRP) Pab Gt x ChI1906-10: IgY (HRP) Pab Rb x ChI1906-12: IgY (FITC) Pab Rb x ChI1906-14: IgY (AP) Pab Rb x Ch
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes mouse Vesicular Acetylcholine Transporter. Species Crossreactivity: rat. No significant sequence homology is seen with other neurotransmitter transporter.
Isotype：IgY
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：A 20aa peptide mapping within the C-terminus, cytoplasmic domain of rat/mouse VAT conjugated to KLH. Species sequence homology: human-93%, monkey-91%.
