Urate, a naturally occurring product of purine metabolism, is a scavenger of oxidants.
Urate exists primarily as a weak acid (pKa ~5.75).
Urate concentration is quite high (~200-500uM) in human due to loss of uricase gene and the presence of an effective reabsorption system.
Recently an OAT-related transporter, URAT1, has been identified in the kidney that acts as urate-anion exchanger and effectively regulates urate levels.
URAT1/RST/SLC22A12 (mouse 553-aa, human 555-aa, chromosome 11q13, ~40kD) is ~42% identical with OAT4.
Like OAT4, it is predicted to display 12 transmembrane domains with a cytoplasmic N- and C-terminus.
URAT1 is prominent in epithelial cells of proximal tubule of the renal cortex.
Patients with idiopathic renal hypouricemia (lack of blood uric acid) have defects in URAT1 gene.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml
Western Blot: 1-10ug/ml using ECL
Optimal dilutions to be determined by the researcher.
Control Peptide:U2005: Urate Transporter 1, Human (URAT1) (Control Peptide)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
Specificity：Recognizes human URAT1. Species Sequence Homology: Mouse RST/URAT1 (54%). No significant sequence homology is observed with other OAT4 or other proteins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 11aa near the C-terminus of human URAT1 (KLH). Cellular Localization: Cytoplasmic domain.
