Urate, a naturally occurring product of purine metabolism, is a scavenger of oxidants.
Urate exists primarily as a weak acid (pKa ~5.75).
Urate concentration is quite high (~200-500 uM) in human due to loss of uricase gene and the presence of an effective reabsorption system.
Recently an OAT-related transporter, URAT1, has been identified in the kidney that acts as urate-anion exchanger and effectively regulates urate levels.
URAT1/RST/SLC22A12 (mouse 553-aa, human 555-aa, chromosome 11q13, ~60 kD) is ~42% identical with OAT4.
Like OAT4, it is predicted to display 12-TM domains with cytoplasmic N and C-terminus.
URTA1 is prominent is epithelial cells of proximal tubule of the renal cortex.
Patients with idiopathic renal hypouricemia (lack of blood uric acid) have defects in URAT1 gene.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL techniqueELISA: 1:10,000-50,000.
Coat ELISA plate with 1ug/ml control peptide
Optimal dilutions to be determined by the researcher.
Control Peptide: U2005
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes Urate Transporter 1 (URAT1). Peptide Sequence Homology: human URAT1/RST or OAT-4-like protein: 100%; mouse RST/URAT1: 54%.; no significant homology with other OAT4 or other proteins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic 11-aa peptide sequence near the cytoplasmic, C-terminus of human URAT1 ( KLH coupled).
