White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure.
WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis.
Uncoupling proteins (UCP1-5) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate.
BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1).
UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs).
Both amino and C-termini are predicted to be cytoplasmic.
Mouse/rat UCP2 is a 309aa (human 309aa, chromosome 7; ~95% homology) mitochondrial uncoupling protein (1).
It is only 59% homologous with UCP1 found in brown adipose tissues.
UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart and muscle).
UCP2 may play a critical role in energy balance, body weight and thermoregulation.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10ug/ml (ECL)Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissue.
Reported to detect UCP2 in human neuroblastoma cell shy cells
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide: U1621-12: Uncoupling Protein 2, Human, Control Peptide (UCP2, SLC25A8, UCPH)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes human UCP2. No significant homology with UCP1 or UCP3-5.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a 13aa sequence between the 3rd and 4th transmembrane domain of human UCP2 conjugated with KLH. Species Sequence Homology: rat and mouse; 100%, canine, bovine and porcine; 92%
