The regulation of body weight depends upon the calorie intake and expenditure.
It is a very complex and highly regulated process.
It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals.
The product of obese gene (Leptin) may influence many of these processes.
White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure.
WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis.
Uncoupling proteins (UCP1-4) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate.
BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1).
UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs).
Both amino and C-termini are predicted to be cytoplasmic.
UCP1 is a member of the mitochondrial carrier protein family, which includes the ATP/ADP translocator, the phosphate, the 2-oxoglutarate/malate and the citrate (tricarboxylate) carriers.
UCP1 plays an important role in thermoregulation in hibernating animals, neonates, and it contributes to rodent cold- and diet induced thermogenesis.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot:1-10ug/ml using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng U1621-01 UCP2 control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA.
Specificity：Recognizes mouse Uncoupling Protein 2 (UCP2). No significant homology with UCP1 or UCP3-5. Species Sequence Homology: rat and hamster:100%; human, porcine, canine and bovine: 86%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A 14 amino acid peptide sequence near the C-terminus of mouse UCP2 (KLH).
