The regulation of body weight depends upon the calorie intake and expenditure.
It is a very complex and highly regulated process.
It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals.
The product of obese gene (Leptin) may influence many of these processes.
White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure.
WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis.
Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate.
BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1).
UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs).
Both amino and C-termini are predicted to be cytoplasmic.
UCP1 is a member of the mitochondrial carrier protein family, which includes the ATP/ADP translocator, the phosphate, the 2-oxoglutarate/malate and the citrate (tricarboxylate) carriers.
UCP1 plays an important role in thermoregulation in hibernating animals, neonates, and it contributes to rodent cold- and diet induced thermogenesis.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:U1620-15: Uncoupling Protein 1, Mouse, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes the mouse Uncoupling Protein 1. The UCP1 peptide has no significant homology with UCP2-5.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：A 19 amino acid peptide sequence at the C-terminus of the mouse (rat) UCP1 (KLH). Species crossreactivity: 100% -rat, 94% -golden hamster, 88% -porcine and 78% -human, rabbit and canine UCP1.
