84-1049-33　［取扱停止］Uncoupling Protein 1 (UCP1) 100ul　U1620-09

特徴

• The regulation of body weight depends upon the calorie intake and expenditure.
• It is a very complex and highly regulated process.
• It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals.
• The product of obese gene (Leptin) may influence many of these processes.
• White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure.
• WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis.
• Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate.
• BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1).
• UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs).
• Both amino and C-termini are predicted to be cytoplasmic.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000.
• An antibody made to this epitope has detected approx.
• 32kD protein in brown adipose tissues (3)
• ELISA: 1:10,000-1:100,000 using 50-100ng UCP1 control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide:U1620-05 Uncoupling Protein 1, Mouse Control Peptide
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.