Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity.
An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity.
High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity.
In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub).
More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned.
The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti.
Agouti protein (132 aa in human) is normally expressed in skin but its ubiquitous expression causes obesity.
Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1).
However, recombinant Agouti protein also antagonizes the MC2R and MC4R.
AGRP (132 aa in human, chromosome 16q21) is normally expressed in adrenal and hypothalamus.
AGRP levels are increased several folds in ob/ob mice.
AGRP is a strong antagonist of MC3R and MC4R.
Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: 1:10,000-50,000 using 50-100ng control peptide/well.
Immunohistochemistry & Immunofluorescence: We recommend the use of affinity pure antibody at 10-50ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：The human peptide has no significant homology with mouse tubby, Agouti, AGRP and TULP1. Antibody crossreactivity in various species is not known. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 21 aa peptide sequence from the middle of human TULP2 (1). Peptide was coupled to KLH
