Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity.
An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity.
High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity.
In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
ELISA: 1:10,000-100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:T9152-50A: Tubby, Mouse Control Peptide (TULP, Tubby-like Protein, Tub Homolog)
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes mouse Tubby. Likely to crossreact with mouse and human Tubby and human TUB (506aa). No homology is seen with Agouti, AGRP, TULP1 and TULP2.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A synthetic peptide corresponding to aa126-144 near the N-terminus of mouse Tubby (KLH). Species Sequence Homology: human tubby and human tub homolog. 95%
