Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity.
An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity.
High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity.
In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub).
More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned.
The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti.
Agouti protein (132 aa in human) is normally expressed in skin but its ubiquitous expression causes obesity.
Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1).
However, recombinant Agouti protein also antagonizes the MC2R and MC4R.
AGRP (132 aa in human, chromosome 16q21) is normally expressed in adrenal and hypothalamus.
AGRP levels are increased several folds in ob/ob mice.
AGRP is a strong antagonist of MC3R and MC4R.
Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:5000-25,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:T9152-30: Tub homolog Protein, Human
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.1% sodium merthiolate, before the addition of glycerol to 40%.
Specificity：Recognizes human Tub homolog. The human peptide has no significant homology with mouse tubby, Agouti, AGRP, TULP1 and TULP2.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 19 aa peptide sequence near N-terminus of human Tub homolog (31-48 aa). Coupled to KLH
