84-1049-19　Thioredoxin (fusion protein) 100ul　T5010-10

特徴

• Thioredoxin catalyzes disulfide exchange in proteins via an oxidation/reduction reaction mechanism and is used for apoptosis inhibition in human PBL blasts and Jurkat T-cells.
• Thioredoxin also functions as a catalyst for filamentous phage assembly and as an accessory protein for phage T7 DNA Polymerase.
• Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices.
• Therefore, tags enable a selective identification and purification of the protein of interest.
• Eukaryotic genes are often cloned into E.
• coli thioredoxin gene, resulting in the expression of a desired protein as a fusion hybrid with thioredoxin (1).
• Thioredoxin is a small electron transport protein that serves as hydrogen door in the enzymatic reduction of ribonucleotides to deoxyribonucleotides (1).
• Thioredoxin from E.
• coli consists of a single polypeptide of 108 aa (~11.7kD).
• The gene encoding Thioredoxin has been clones into several plasmids and serves as a fusion partner for the expression of other clones genes.
• The system often produces high expression of soluble fusion proteins in the E.
• coli cytoplasm.
• The fusion proteins may also fold correctly with biological activity.
• Anti-Thioredoxin antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysate, using immunoaffinity chromatography (1) or used in immunoprecipitation.
• Anti- thioredoxin can also be used for the immunocytochemical detection of thioredoxin in cells and tissues that express transfected bacterial thioredoxin gene (1).
• Ant-thioredoxin may be used in various immunoassays to identify the expression of thioredoxin fusion protein.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL.
• Antibodies react with native and denatured THRx-tag containing proteins
• ELISA: 1:10-50K using 50-100ng control antigen/well)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.