The 3' ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere.
The telomeric RNA contains a sequence complementary to TTAGGG.
In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase.
Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death.
Approximately 4.8kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities
Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD.
p123 is a homolog of yeast Est2 (Essential for Telomerase activity).
Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132aa; mouse Est2 1122aa; ~127kD).
hEST2 was localized in the nucleus.
Yeast telomerase is 988aa protein.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml.
Control peptide, #T2399-80, can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes yeast telomerase.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Ye
Immunogen：Synthetic peptide corresponding to 23aa near the N-terminus of yeast telomerase/Est2 conjugated with KLH.
