84-1049-14　Telomerase (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT) 100ul　T2399-85

特徴

• The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
• Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere.
• The telomeric RNA contains a sequence complementary to TTAGGG.
• In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase.
• Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death.
• Approx.
• 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.
• Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD.
• p123 is a homolog of yeast Est2 (Essential for Telomerase activity) and show 20% homology.
• Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned.
• Est2 is associated with telomerase activity and TP1.
• Mouse/rat and human TP1 are 2627 and 2629 aa proteins, respectively.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-50,000 for neat serum.
• Control peptide (Catalog #T2399-80) can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.