The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere.
The telomeric RNA contains a sequence complementary to TTAGGG.
In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase.
Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death.
Approx.
4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml.
Using ECLELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Control Peptide:T2399-65: Telomerase Protein, Mouse, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes mouse telomerase 1. Species sequence homology: rat, 100%; human, 89%
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A 19aa peptide sequence in the middle portion of mouse TP1 (KLH).
