The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
Telomerase is an unusual RNA-dependent DNA polymerase that uses an RNA component to specify the addition of telomere.
The telomeric RNA contains a sequence complementary to TTAGGG.
In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase.
Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death.
Approximately 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.
Most recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doublings
Purification of telomerase from the ciliate also revealed two proteins of 43 and 123kD.
p123 is a homolog of yeast Est2 (Essential for Telomerase activity).
Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; mouse Est2 1122 aa; ~127kD).
hEST2 was localized in the nucleus.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
The antibody has detected ~120-130kD band from several tumor cell lines.
An antibody made to a region near to the hESt21 epitope recognized ~125kD band in human and mouse tissues (2).
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide: T2399-60
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Sold under license from Geron
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human Telomerase.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 21 amino acid peptide sequence mapping in the middle of human Est2 (KLH coupled). No significant sequence homology with mouse Est2.
