84-1049-07　Telomerase (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT) 100ul　T2399-20

特徴

• The 3' ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
• Telomerase is an unusual RNA-dependent DNA polymerase that uses an RNA component to specify the addition of telomere.
• The telomeric RNA contains a sequence complementary to TTAGGG.
• In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase.
• Many mammalian cells do not express telomerase resulting in shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death.
• Approximately 4.8 kb of telomeric DNA is lost with each cell division resulting in large number of chromosomal abnormalities.
• Most recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~20 doubling.
• Purification of telomerase from the ciliate also revealed two proteins of 43 and 123kD.
• p123 is a homolog of yeast Est2 (Essential for Telomerase activity).
• Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; mouse Est2, 1122 aa; ~127kD)
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-50,000Western Blot (ECL): 1:1000-5000 using ECLOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.