The 3' ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
Telomerase is an unusual RNA-dependent DNA polymerase that uses an RNA component as a template to lengthen telomeres.
The telomeric RNA contains a sequence complementary to TTAGGG.
In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase.
Many mammalian cells do not express telomerase resulting in the shortening of telomere with each cell division, and ultimately causing chromosomal instability, aging and cell death.
Recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~20 doublings.
Purification of telomerase from the ciliate also revealed two proteins of 43 and 123kD.
p123 is a homolog of yeast Est2 (Essential for Telomerase activity).
Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132aa; mouse Est2, 1122aa; ~127kD).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/mlWestern Blot: 1-10ug/ml using ECL.
Mouse telomerase detected in testes, thymus and spleen.
Optimal dilution to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity：Recognizes Telomerase from mouse FM3A at ~125kD. Also reacts with human immortal cell line 293.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 15aa in the mid-region of mouse Telomerase. Species Sequence Homology: human and rat: 100%. No significant homology with yeast Telomerase.
