TRF1 is a negative regulator of telomere length maintenance (1).
TRF1 has a number of biochemical similarities to Rap1p, a distantly related DNA-binding protein that functions at telomeres in yeast (1,2).
Like Rap1p, TRF1 requires two Myb motifs for DNA binding.
Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified.
Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (3).
Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (3,4).
Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation.
Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation.
Tin2 localizes to telomeres and is essential for proper regulation of telomere length.
TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase.
Recently, it has been shown that TRF1 expression is significantly elevated in patients with acute lymphoblastic leukemia (ALL) compared to those in acute myeloid leukemia (AML) (5).
In this study, TRF1 expression tended to be higher in patients without telomere shortening and in those with hTERT expression suggesting that TRF1 may act to monitor telomere length under the condition of up-regulated telomerase activity in some neoplastic cells.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
A band of 95-110kD has been detected in brush border membrane (2)
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：The 20 AA human control peptide has no significant sequence homology with TRF2. We recommend the use for the use mouse TRF1. Actual crossreactivity of antibodies in various species has not been studied
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 20 AA Peptide sequence near the C-terminus of human TRF1 (2). The peptide was coupled to KLH
