84-1049-01　Telomeric Repeat Binding Factor 1 (TRF1, RAP1) 100ul　T2395-01

特徴

• The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
• Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere.
• The telomeric RNA contains a sequence complementary to TTAGGG.
• In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase.
• Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death.
• Approx.
• 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
• A band of 95-110kD has been detected in brush border membrane (2)
• ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
• Control peptide can be used to coat ELISA plates at 1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.