Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Immunoprecipitations: We recommend 5-10 ul neat serum or 1-10ug affinity pure antibody per 100ug of tissue
Immunohistochemistry & Immunofluorescence: We recommend the use of affinity purified antibody at 2-20ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：The rat immunogenic peptide shows 100% homology to mouse and 90% to human and canine Taurine transporter (1). The peptide shows no significant homology with other known proteins. The Taurine transporter antibody crossreactivity in other species is not known. The appropriate control immunogenic peptides are also available to confirm specificity of antibodies.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：20aa peptide near the carboxy terminal cytoplasmic region of rat TAU-1
