Higher vertebrates are believed to possess at least five basic tastes: Sweet, bitter, sour, salty, and unami (the taste of monosodium glutamate).
Taste receptor cells that may selectively reside in various parts of the tongue and respond to different tastants and perceive these taste modalities.
Circumvallate papillae, found at the very back of the tongue, are particularly sensitive to biter substances.
Foliate papillae, found at the posterior lateral edge of the tongue, are sensitive to sour and bitter.
Fungiform papillae at the front of the tongue specialize in sweet taste.
Recently, two novel taste receptors, TR1 and TR2, have been cloned with distinct topographical distribution in taste receptor cells and taste buds.
TRs are members of a new group of 7 TM domain containing GPCR distantly related to other chemosensory receptors (Ca+-sensing receptor (CaSR, a family of putative hormone receptor (V2R), and metabotropic glutamate receptors).
TR1 is expressed in all fungiform taste buds, whereas TR2 localized to the circumvallate taste buds.
Both receptors do not colocalize with gustducin.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide: T1024-01B Taste Receptor 1, Rat Control Peptide (TR1)
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a powder lyophilized from a solution containing PBS and 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes rat TR1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：KLH-conjugated 24-amino acid peptide sequence near the C-terminus of rat TR1. This sequence has no significant homology with TRS or gustducin or pheromone receptors. Species sequence homology: mouse (100%); human (87%).
