84-1048-94　［取扱停止］Taste Receptor 1 (TR1) 100ul　T1024-01C

特徴

• Higher vertebrates are believed to possess at least five basic tastes: Sweet, bitter, sour, salty, and unami (the taste of monosodium glutamate).
• Taste receptor cells selectively reside in various parts of the tongue and respond to different tastants and perceive these taste modalities.
• Circumvallate papillae, found at the very back of the tongue, are particularly sensitive to biter substances.
• Foliate papillae, found at the posterior lateral edge of the tongue, are sensitive to sour and bitter.
• Fungiform papillae at the front of the tongue specialize in sweet taste
• Recently, two novel taste receptors, TR1 and TR2, have been cloned with distinct topographical distribution in taste receptor cells and taste buds.
• TRs are members of a new group of 7 TM domain containing GPCR distantly related to other chemosensory receptors (Ca+-sensing receptor (CaSR), a family of putative hormone receptor (V2R), and metabotropic glutamate receptors).
• TR1 is expressed in all fungiform taste buds, whereas TR2 is localized to the circumvallate taste buds.
• Both receptors do not colocalize with gustducin.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:5000-25,000.
• Control peptide can be used to coat ELISA plates at 1ug/ml
• Western Blot: 1:500-1:2500
• Optimal dilutions to be determined by the researcher.
• Control peptide: T1024-01B
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• .
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.