84-1048-93　［取扱停止］Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase, TANK2, TNKL) 100ug　T0900-25

特徴

• The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
• Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere.
• The telomeric RNA contains a sequence complementary to TTAGGG.
• Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling.
• Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate.
• Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity.
• Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere.
• The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml.
• Using ECL.
• TANK1 ~130kD
• ELISA: 0.5-1ug/ml.
• Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Control Peptide:T0900-08: Tankyrase, Human (TRF1 interacting ankyrin-related ADP-ribose polymerase, TANK2, TNKL)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.