The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere.
The telomeric RNA contains a sequence complementary to TTAGGG.
Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling.
Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate.
Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity.
Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere.
The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers.
TANK1 has 24 ankyrin repeats in TRF-1 interacting domain near the N-terminus.
The ANK domain is followed another protein interaction motif called the sterile alpha-module (SAM).
The C-terminal region of TANK1 contains the PARP activity.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000
ELISA: 1:10,000-50,000.
T0900-08 Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.Reconstitute in 100ul of sterile PBS.
Specificity：Recognizes human Tankyrase.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide,14aa corresponding to the area between the ANK15 and SAM domain of human TANK2 coupled to KLH (UniProt #Q9H2K2).
