The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication.
Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere.
The telomeric RNA contains a sequence complementary to TTAGGG.
Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling.
Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate.
Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity.
Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere.
TANK1 is alternatively spliced to isoform 1 and 2 (missing 644-1327).
The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers.
TANK1 has 24 ankyrin repeats in TRF-1 interacting domain near the N-terminus.
The 33-aa ANK repeat motif mediates protein-protein interactions.
The ANK domain is followed another protein interaction motif called the sterile alpha-module (SAM).
The C-terminal region of TANK1 contains the PARP activity.
TANK1 uses its ANK domain to bind TRF1 and its PARP domain to ADP-ribosylate itself and TRF1, and thereby inhibiting the ability of TRF1 to bind telomere.
The homology between tankyrase and PARPs is limited to catalytic domain.
Takyrase-1 is expressed in many tissues and targeted to various intracellular compartments.
Tanyrase-1, devoid of NLS (nuclear localization signal), is translocated to telomere (nucleus) through binding of its ANK domain to TRF1.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
TANK1 ~142kD.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：The human TANK12 control peptide sequence is not found in TANK2. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 17-aa Peptide sequence within the N-terminal (after the 1st poly-pro) domain of human TANK1 (1) was synthesized, coupled to KLH
