84-1048-80　Stearoyl CoA Desaturase 1 (SCD1) 100ug　S7900-05

特徴

• Stearoyl-CoA desaturase (SCD, also known as Delta-9 desaturase) is an iron containing membrane enzyme.
• It catalyzes the rate limiting step in the biosynthesis of mono-unsaturated fatty acids.
• The enzyme inserts a cis double bond between the C9 and the C10 positions in the acyl-CoA derivatives of saturated fatty acids such as palmitic acid and stearic acids to produce palmitoleoyl- and oleoyl-CoA, respectively.
• Mono-unsaturated fatty acids play an important role in many processes, including energy metabolism (triacylglycerol storage in adipose tissues), anti-oxidation, lipid acivated signal transduction, apoptosis, mitogenesis in certain tumors, and activate certain hormone receptors.
• SCD genes are tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature.
• Three SCD genes SCD1, SCD2 and SCD3 have been identified in mouse.
• All three genes have been mapped at mouse chromosome 19 D2.
• In human, there is only one structural SCD-gene (chromosome 10) encoding two SCD transcripts has been identified.
• The SCD-gene products (SCD-1, 2 and 3) are integral membrane proteins with four TM domains containing a novel sterol regulatory element binding protein (SREBP) binding site.
• SREBP regulates enzymes involved in cholesterol, triglyceride and fatty acid metabolism.
• The SCD enzymes have a 30aa N-terminal sequence responsible for their rapid degradation and short half life of 3-4 hours.
• The enzyme SCD-1 is constitutively expressed in adipose tissue and is induced in liver in response to fat free, high carbohydrate diet.
• The SCD-1 expression is higher in female than male mice.
• Although, the enzyme is expressed in undifferentiated sebocytes of skin at a lower level than SCD-3, its expression is crucial to the differentiation of sebocytes.
• The loss of gene expression results in asebia characterized by hypoplastic sebaceous and meibomian glands, flaky skin and alopecia.
• The repression of RNA levels and enzyme activity of hepatic SCD-1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation.
• Inhibition of SCD-1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/ml (Coat with 1ug/ml control peptide)
• Western Blot: 1-10ug/ml for ECL
• Optimal dilutions to be determined by the researcher.
• Control Peptide: S7900-01
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.