The kidneys play a major role in the regulation of glucose levels.
Kidneys filter approximately180 grams of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules.
Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucose co-transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs).
At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species.
Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains.
SGLT1-3 genes code for protein of approx 659-672 residues (calculated size of ~75kD).
Both N and C-termini are predicted to be extracellular.
There is approximatelu 60-70% homology between SGLT1-3.
SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner.
SGLT1 does not discriminate a-MDG, glucose, and galactose.
SGLT2/3 do not transport D-galactose efficiently.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml.
S1010-87L7 (control peptide) can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide: S1010-87L7
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS containing 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human SGLT-3.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic 15-aa peptide sequence from the C-terminal, cytoplasmic domain 6 of Human SGLT-3 (KLH). No significant sequence homology exists with other SGLTs.
