Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
A band of 95-110kD has been detected in brush border membrane (2)
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：porcine
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The porcine peptide sequence is 61% conserved in human SGLT3. No significant sequence homology exists with other SGLTs. For mouse SGLT3, we recommend the use antibody and SG-32 for human SGLT-3 that are made to the mouse and human SGLT-3 peptide, respectively. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity This antibody has previsouly been available as for porcine SGLT-2. The same antibody is now reclassified as porcine SGLT-3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Po
Immunogen：A 14 AA peptide sequence from the N-terminal, cytoplasmic domain 1 of pig SGLT-3 (1) was synthesized, coupled to KLH
