The kidneys play a major role in the regulation of glucose levels.
Kidneys filter approx.
180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules.
Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucoseco transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs).
At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species.
Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains.
SGLT1-3 genes code for protein of approx 659-672 residues (calculated size of ~75kD).
Both N and C-termini are predicted to be extracellualr.
There is approx 60-70% homology between SGLT1-3.
SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner.
SGLT1 does not discriminate a-MDG, glucose, and galactose.
SGLT2/3 do not transport D-galactose efficiently.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml.
Coat ELISA plates at 1ug/ml with control peptide
Western Blot: 1-10ug/ml using ECL.
Detects a band at ~70-77kD
Optimal dilutions to be determined by the researcher.
Control Peptide:S1010-86E: SGLT-1, Human, Control Peptide (Sodium Glucose Transporter 1)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes human SGLT-1. No significant sequence homology exists with other SGLTs. Species sequence homology (56%): mouse, rat. For mouse and rat, we recommend S1010-86D or S1010-86G.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 16aa sequence from the C-terminal, cytoplasmic domain 6 of human SGLT-1 conjugated to KLH.
