The kidneys play a major role in the regulation of glucose levels.
Kidneys filter approx.
180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules.
Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucoseco transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs).
At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species.
Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains.
SGLT1-3 genes code for protein of approx 659-672 residues (calculated size of ~75kD).
Both N and C-termini are predicted to be extracellualr.
There is approx 60-70% homology between SGLT1-3.
SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner.
SGLT1 does not discriminate a-MDG, glucose, and galactose.
SGLT2/3 do not transport D-galactose efficiently.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL.
SGLT-1 is ~70-77kD
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:S1010-86C: SGLT-1, Rat Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes rat SGLT-1. No significant sequence homology exists with other SGLTs. Species Sequence Homology: mouse; 93%, human 50%. For human, we recommend S1010-86H.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 16aa from the N-terminal, cytoplasmic domain of rat SGLT-1 conjugated to KLH.
