Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000.
Control peptide can be used to coat ELISA plates at 1ug/ml
Western Blot: 1:1000-1:5000 using ECL.
Detects a band of ~70-77kD
Optimal dilutions to be determined by the researcher.
Control Peptide:S1010-86C: SGLT-1, Rat Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes rat SGLT-1. No significant sequence homology exists with other SGLTs. Species sequence homology: mouse 93% and human 50%. For human, we recommend S1010-86H or S1010-86K.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 16aa from the N-terminal, cytoplasmic domain of rat SGLT-1 conjugated to KLH.
