84-1048-62　SGLT-1 (Sodium Glucose Transporter 1) 100ul　S1010-86A

特徴

• The kidneys play a major role in the regulation of glucose levels.
• Kidneys filter ~180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules.
• Typically, glucose is first absorbed within epithelium by a specific transporter protein, sodium glucose co-transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes bya facilitated sugar transporter (GLUTs).
• 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species.
• Individual members of this family have identical predicted secondary structures with up to 14 transmembrane domains.
• SGLT1-3 genes code for protein of ~659-672 residues (75kD).
• Both N and C termini are predicted to be extracellular.
• There is ~60-70% homology between SGLT1-3.
• SGLTs transport a-methyl-D-glucoside (a-MDG), a non metabolized model substrate, in Na-dependent manner.
• SGLT1 does not discriminate a-MDG, glucose, and galactose.
• SGLT2/3 do not transport D-galactose efficiently.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000, ~70-77kD
• ELISA: 1:10,000-1:50,000.
• Coat ELISA plates with control peptide (S1010-86F) at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.